Urea solubility

Sample type:

plasma samples

Uses:

Traditionally, the solubility of fibrin clot in concentrated urea, acetic acid or monochloroacetic acid solution was used to screen for FXIII deficiency.

Interfering factor:

The clot solubility test is not standardized, and careful consideration is mandatory. The sensitivity of this method is dependent upon fibrinogen level, the reagent used for clotting (Ca2+, thrombin, etc.), and the solubilizing agents (MCA acid, acid acetic, or 5 M urea). Combination of acetic acid/thrombin, acetic acid/calcium, urea/calcium, and urea/thrombin can modify the relative sensitivity of clot solubility assays

Pre-analytical error:

• Inappropriate or wrongly handled procedures.

• Problems may arise during the collection of blood specimens

• Some factors can alter the result of a sample constituent after collection during transportation, preparation and storage.

Corrective action

Proper sample collection and handling.

Post analytical errors:

• Check instrument settings

• Assure that all preventive maintenance has been done

• Carry out troubleshooting functions called for in the SOP

• Call for equipment support

Corrective action:

• Assure that the prescribed preventive maintenance for the instrument is performed