Testosterone total,free,bioavailable

Sample type:

Serum (Red tube)

Uses :

Evaluation of gonadal hormonal function, because Testosterone is the major androgenic hormone. It is responsible for the development of the male external genitalia and secondary sexual characteristics. In females, its main role is as an estrogen precursor. In both genders, it also exerts anabolic effects and influences behavior.

Precautions

• Sample should be on red tube, Serum gel/SST are not acceptable .

• Testosterone testing should be performed between 7 a.m. and 10 a.m .which is around when levels are normally the highest.

Some doctors may require you to prepare for the test by fasting, which means avoiding eating or drinking anything but water for a few hours before the test.

• It’s very important to know patient’s age and sex.

-It’s preferred to collect specimen in the morning.

Interfering factors

Early-morning testosterone levels in young male individuals are, on average, 50% higher than p.m. levels. Reference values were established using specimens collected in the morning

• free testosterone measurements by calculation using algorithms based on the law of mass action, which require total testosterone, SHBG ,and albumin concentration have excellent correlations with physical separation measures.

Pre-analytical errors

1 – unlabeled or mislabeled specimen

2 – if the sample was hemolysis or lipemic .

The Corrective action

Reject the sample and another one is obtained.

Reject the sample and another sample is obtained

Post analytical errors

send wrong results to the patient .

The Corrective action

you must communicate with the patient and apologize for this mistake and sent the right report

Reference rang

Testosterone total , males

10 -11 years: 2-57 ng/dl

12-13 years: 7- 747 ng/dl

18- 39 years: 400- 1080 ng/dl

60 years and older :350-890 ng/dl

Females

16-17 years: 8-63 ng/dl

18-30 years: 11- 59 ng/dl

Testosterone, free male

7- 9 years: 0,1 - 0,9 pg/ml

14- 15 years: 3 - 138 pg/ml

16- 17 years: 38- 173 pg/ml

18 years and older: 47-244 pg/ml

Females

10- 11 years: 0,1 - 3.5 pg/ml

12 - 13 years: 0,9 - 6.8 pg/ml

18 -30 years: 0,8 -7.4 pg/ml

Testosterone bioavailable

Male

14-15 years: 10-337 ng/dL

16-17 years: 35-509 ng/dL

18 years and older: 131-682 ng/dL

Tanner Stage IV: 40-485 ng/dL

Tanner Stage V: 124-596 ng/dL

Female

12-13 years: 1.7-18.8 ng/dl

16-17 years: 3.3-22.6 ng/dl

18-30 years: 2.2- 20.6 ng/dl

Tanner Stage l : 3 -5.5 ng /dl

Tanner Stage ll: 1.2 -15 ng/dl

Tanner Stage lll: 3.8 - 28 ng/dl