Progesterone
(PGSN)

Sample type

Serum (preffered)

Plasma (lithium heparin)

Uses

-Detection of ovulation in the evaluation of the function of the corpus luteum.

-Monitoring patients having ovulation during induction with hCG, human menopausal,gonadotropin, FSH/LH-releasing hormone, or clomiphen.

-To evaluate patients at risk for early abortion.

Precautions

-Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.

-Centrifuge specimen within 2 hours of collection. Remove serum aliquot into a screw-capped round bottom plastic vial.

Interfering factors

-Taking estrogen and progesterone supplements can affect results.

-Taking biotin supplements (B7,B8, vitamin H, and coenzyme R)can affect results.

Hemolysis -

Pre-analytical errors

1-Gross hemolysis, mislabeled or unlabeled specimens

2- The patient is taking biotin supplements

3-The patient’s history has not been taken carefully and therefore there is information that needs to know and is not available.

The corrective action

1-The sample should be rejected and another sample requested.

2-Do not collect the sample and ask the patient to stop taking biotin supplements for 72 hours.

3-You must communicate with the patient and know all the informations you need. And in the next time the history of the patient should be taken carefully.

Post-analytical errors

1-Writing a wrong result or wrong name in the report

2-If the patient’s gender is written wrong in the report, the reference range was written incorrectly

3-report were sent to incorrect patient

The corrective actions

1-If the report is not delivered to the patient and this error is discovered, the correct result or the correct name must be written, but if the report is delivered to the patient, you must communicate with the patient, apologise to him, and tell him that an error has occurred and replace it with the correct report.

2-If the report is not delivered to the patient and this error is discovered, fix it, but if the report is delivered to the patient, apologise to him, tell him that an error has occurred, and replace it with the correct report. And next time, Be careful when you write down the gender and reference range.

3-Communicate with patient ,apologizing for the error, and providing him with the correct report

Reference range

Male: 0.0-0.5 ng/mL

Female:

Follicular phase 0.1-0.9 ng/mL

Luteal phase: 1.8-23.9 ng/mL

Ovulatory phase: 0.1-12.0 ng/mL

Pregnant:

1st trimester : 11.0-44.3 ng/mL

2nd trimester: 25.4-83.3 ng/mL

3rd trimester 58.7-214.0 ng/mL

Postmenopausal: 0.0-0.1 ng/mL