PHOSPHATIDYLGLYCEROL
(PG)

Sample type

Amniotic Fluid

Uses

• Assessing fetal lung maturity

• Determining the ability of fetal lungs to produce sufficient quantities of pulmonary surfactant.

• Predicting the likelihood of the development of respiratory distress

syndrome if the fetus were delivered.

Precautions

Do not centrifuge

Amniotic specimens must be free of blood and meconium contamination.

Interfering factors

AmnioStat-FLM is not subject to artifacts associated with other lung surfactant tests.

TLC test can produce false-positive test results with meconium contamination and vaginal fluid contamination.

The absence of PG or low levels of PG cannot dependably predict the presence of RDS.

Diabetes, regardless of glucose control, delays PG production.

Pre-analytical errors

1. If the sample was not collected with the correct instructions.

The corrective action

The sample should be rejected and another sample requested.

1. Gross hemolysis, mislabeled or unlabeled specimens

The corrective action

The sample must be rejected and another sample be obtained

Post-analytical errors

I. reports were sent to the incorrect patient

The corrective action

communication with patient, apologizing for the error, and providing him with the correct report

II. write the wrong name in the report or the wrong results.

The corrective action

If the report is not delivered to the patient and this error is discovered, the correct result or the correct name must be written, but if the report is delivered to the patient, you must communicate with him, apologies to him, and tell him that an error has occurred and replace it with the correct report.

Reference range

Immature: <15,000/mcL

Indeterminate: 15,000-50,000/mcL

Mature: >50,000/mcL