Mixed lymphocytes culture

Sample type:

whole blood with heparin anticoagulant and tissue slices.

Uses:

is technique used to assess whatever an individual is going to undergoing an immune response against another individual cells .

The test is important for determining histocompatibility between donor and recipient in the transplantation of bone marrow and organs.

Precaution :

no test preparation is needed.

Pre - analytical errors:

Contaminated sample

Corrective action :

Proper sample collection and handling

Post analytical errors:

1- Writing wrong name in the report

2- Writing a wrong result in the report

The corrective action

1/Make sure you write the correct name in the report or use codes that contain all patient data .

2-If the report is not delivered to the patient and this error is discovered the correct result must be written, but if the report is delivered to the patient must communicate with him and tell him that an error has occurred and replace with the correct report.

Reference values:

Negative : Lymphocytes from incompatible individuals will stimulate each other to proliferate significantly

Positive : Lymphocytes from compatible individuals will not .