Lymphocyte surface marker analysis
(LSM)

Sample type:

EDTA

Uses:

This test measures percentages and absolute numbers of lymphocytes, CD3 T cells, CD4 T cells and CD8 T cells and in lymphocyte subsets also B cells and NK cells.

Precaution :

A blood sample drawn from a vein for testing

Interfering factor :

Specimen must arrive within 48 hours of draw. Draw and package specimen as close to shipping time as possible.

Pre - analytical errors:

Sample rejected due to:

1/Gross hemolysis

2/Gross lipemia

Corrective action :

Proper sample collection and handling

Post analytical errors:

1- Writing wrong name in the report

2- Writing a wrong result in the report

The corrective action

1/Make sure you write the correct name in the report or use codes that contain all patient data .

2/If the report is not delivered to the patient and this error is discovered the correct result must be written, but if the report is delivered to the patient must communicate with him and tell him that an error has occurred and replace with the correct report.

Reference range :

Age range	CD3+ T cells	CD19+ B cells	CD4+ T cells	CD8+ T cells	CD16/CD56+ NK cells	CD4/CD8 Ratio
Neonatal (cells/ul)	600 – 5000	40 – 1100	400 - 3500	200 - 1900	100 - 1900	1.0 - 2.6
1 wk - 9 months (cells/ul)	2300 – 7000	600 – 3000	1400 – 5300	400 - 2200	100 - 1400	1.3 - 6.3
9 - 24 months (cells/ul)	1400 – 8000	600 – 3100	900 - 5500	400 - 2300	100 - 1400	0.9 - 3.9
2 - 5 years (cells/ul)	900 – 4500	200 – 2100	500 - 2400	300 - 1600	100 - 1000	0.9 - 2.9
5 - 10 years (cells/ul)	700 – 4200	200 – 1600	300 - 2000	300 - 1800	90 - 900	0.9 - 2.6
10 - 16 years (cells/ul)	800 – 3500	200 – 600	400 - 2100	200 - 1200	70 - 1200	0.9 - 3.4
Adult (cells/ul)	690 – 2540	90 - 660	410 - 1590	190 - 1140	90 - 590	1.0 - 3.6