Euglobulin lysis time
(ELT)

Sample type:

blood sample, plasma

Uses:

a test measures overall fibrinolysis.

precautions

• When drawing the sample, be careful not to rub the area over the vein too vigorously, pump the fist excessively, or leave the tourniquet in place too long.

• Avoid excessive probing during venipuncture, and handle the sample gently.

• If a blue-top tube is used, mix the sample and anticoagulant thoroughly. If a chilled tube containing 0.5 ml sodium oxalate is used, mix the sample and preservative thoroughly, pack the sample in ice, and send it to the laboratory immediately.

Interfering factor:

• Prolonged tourniquet constriction, vigorous vein preparation, or excessive pumping of the fist (decrease).

• Hemolysis due to excessive probing at the venipuncture site or to rough handling of the sample.

• Failure to place the collection tube and sample on ice

• Fibrinogen level < 100 mg/dl (possible decrease)

Pre-analytical error:

• Coagulation samples should not be transported or stored on ice

• Coagulation samples for platelet function studies must be maintained at room temperature

• Gentle inversion (mixing) of sodium citrates tube 5–6 times is recommended. Avoid rigorous shaking or agitation 69 3.2% sodium citrate is the citrate concentration of choice

• If the syringe technique is to be used, then a syringe of less than 25 mL (preferably 10 mL) using a “butterfly” needle apparatus

Post analytical errors:

• Check instrument settings

• Assure that all preventive maintenance has been done

• Carry out troubleshooting functions called for in the SOP

• Call for equipment support

Corrective action:

• Assure that the prescribed preventive maintenance for the instrument is performed

Reference Ranges

Hyperfibrinolysis: <60 minutes

Hypofibrinolysis: >240 minutes